Cadmium Chloride and Silver Nitrate Affect the Gene Expression, Stevioside, and Rebaudioside: A Production in Stevia rebaudiana (Bert.)

Stevia rebaudiana (Bert.) produces steviol glycoside sweeteners that are sweeter than sucrose. Stevioside and rebaudioside A important glycosides used in food production. This study was conducted to investigate the effect of different concentrations cadmium chloride (0, 20, 40, 60 80 mg/L) silver nitrate 15, 30, 45 sampling times on stevioside production Ent-kaurenoic acid 13-hydroxylase, uridine diphosphate-glycosyltransferase 74G1 76G1 genes expression vitro conditions. The results showed application decreased Among various chloride, highest were respectively 39.76 mg/g 2.27 mg/L dry weight 96 72 h after elicitation with 20 chloride. nitrate, amounts 33.33 2.19 obtained at control condition for 24 h. In all three increased but gene 13-hydroxylase diphosphateglycosyltransferase expression. correlation analysis not correlated expression, while inversely 76G1. Also, positive correlations observed between synthesis A.